The Lamborghini Islero, which made its first appearance at the 1968 Geneva Auto Show was manufactured from 1968 to 1969. The car with the Lamborghini V12 engine came as the substitute for the 400GT. The car was named after the Miura Bull that killed Manuel Rodriguez, the famous matador in August 1947. The chassis of the Islero was designed by Carrozzeria Marazzi as Carrozzeria Touring had gone bankrupt. Lamborghini, the Italian automaker also manufactured a car called Miura between the years 1966 to 1973.

The design of the car was similar to the 400GT, however there were some alterations done to the track so as to accommodate wider tires. The Islero was also better than its predecessor in terms of its outward visibility, spacious interior and an improved soundproofing. The 4L V12 engine of the car had a 325 brake horsepower. It also had disc brakes and independent suspension. With a five speed transmission, the car could reach a top speed of 154mph and could accelerate from 0 to 60 in just 6.4 seconds.

The Islero S was later released in 1969 with some improvements made to the earlier model. The car had some major modifications done to its styling as well as the makeup of the overall body. The Islero S reached top limits of 161mph and took just 6.2 seconds to reach 60 from scratch. Compared to the 125 Islero, only 100 Islero S was manufactured during its time of production. The Islero S also appeared in the Hollywood thriller The Man Who Haunted Himself.